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Atlas of breast cancer early detection

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Breast pathology – Histopathology of the breast – Processing of tissue for histopathology


Breast tissues must be handled and fixed with care to enable the pathologist to give a complete and accurate diagnosis. A specimen received by the histopathology laboratory goes through several procedures before its microscopic features are evaluated by a pathologist. The processing of tissue at the histopathology laboratory includes:
  • grossing of the specimens;
  • obtaining appropriate tissue sections;
  • processing of the tissue through a series of chemical reagents;
  • embedding in paraffin;
  • cutting thin sections and mounting on slides; and
  • staining with H&E.
These techniques are described in the next sections.


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