Instruments, consumables, and setup required for examination after application of acetic acid Ė Preparation of 5% dilute acetic acid
Although a concentration between 3% and 5% is recommended for the acetic acid to be used, we recommend using 5% acetic acid. This ensures that the lesions are visualized distinctly and does not cause any vaginal irritation.
Ingredients required for preparation of 100 mL of 5% acetic acid:
Apparatus and consumables required:
Storage: Store in a tightly capped bottle. Unused acetic acid should be discarded at the end of the day. Acetic acid is hygroscopic and attracts moisture from the atmosphere. This causes the solution to get diluted easily. Therefore, the solution should be freshly prepared daily before the clinic starts.
Caution: It is important to dilute the glacial acetic acid, because undiluted acetic acid causes a severe chemical burn if applied to the epithelium. If a burn occurs, wash immediately with a liberal amount of clean water. It is always a good practice to keep the bottle of glacial acetic acid clearly labelled and keep it away from the room where the examination is performed.