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| Harris haematoxylin: |
Proportions for approximately 2 liters:
- Haematoxylin: 9 g
- Ethanol (absolute): 90 ml
- Potassium alun: 180 g
- Distilled water: 1800 ml
- Mercuric oxide: 4,5 g
- Thymol: 9 g
| Instructions:
- Dissolve 1 g of haematoxylin in 10 ml of absolute ethanol.
- Dissolve 20 g of potassium alun in 200 ml distilled water other gentle heat.
- Before boiling point, add the disloved haematoxylin to the Alun solution (careful of splashing).
- Boil the mixture as quickly as possible for few minutes.
- Slowly add 0,5 g of mercuric oxide (take great care against splashing).
- Quickly cool the mixing in a cold water bath.
- Filter after 24 hours.
- Add 1 g of thymol for conservation purposes.
| | Orange G: |
Proportions for approximately 800 ml:
- Orange G: 4 g
- Distilled water: 36 ml
- 95° ethanol: 760 ml
- Phosphotungstic acid: 0,12 g
| Instructions:
- Dissolve 2 g of orange G in 18 ml distilled water, under gentle heat.
- After cooling, add 380 ml of 95° ethanol.
- Filter.
- Add 0,06 g of phosphotungstic acid.
| | EA 50: |
Proportions for approximately 800 ml :
- Yellow eosin: 2 g
- Light green: 2 g
- Bismarck brown: 0,25g
- Distilled water: 38,25 ml
- 95° ethanol: 1042,5 ml
- Phosphotungstic acid: 1,8 g
- Lithium carbonate
| Instructions:
- Dissolve 2 g of yellow eosin in 18 ml distilled water, and add 380 ml of 95° ethanol.
- Dissolve 2 g of light green in 18 ml distilled water, and add 380 ml of 95° ethanol.
- Dissolve 0,25 g of Bismarck brown in 2,25 ml distilled water, and add 147,5 ml of 95° ethanol.
- Take 398 ml of the light green solution, 398 ml of the eosin solution, 87,5 ml of the brown Bismarck solution.
- Filter the mixture (the 3 sstains together).
- Finally add 1,8 g phosphotungstic acid, 9 drops of a saturated lithium carbonate solution, and 135 ml of 95° ethanol.
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