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Cytopathology of the uterine cervix - digital atlas
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Specimen processing - Lab technique
 
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Post 1
70° ethanol
2 mn or more
Post 2
water
2 mn
Post 3
Harris haematoxylin
4 mn
Post 4
HCl 0,05%*
* For all types of specimen except for the cervical smears where the HCl bath is replaced by a water bath.
1 mn 30 s
Post 5
water
5 mn
Post 6
95° ethanol
2 mn
Post 7
orange G
3 mn
Post 8
95° ethanol
2 mn
Post 9
EA 50
4 mn
Post 10
95° ethanol
2 mn
Post 11-12
Absolute ethanol **
** ** It is important to check the quality of the absolute ethanol regularly because it hydrates quickly.
2 mn minimum
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