Using Human Papillomavirus (HPV) detection tests for cervical cancer screening and managing HPV-positive women – a practical guide / Activity 5

The greatest advantage of self-collection of samples (also known as self-sampling) for HPV tests is that there is no requirement for a pelvic examination by a trained provider. Hence, there is no requirement for an examination table or any special instruments. The women can self-collect sample from her upper vagina either at home or in a clinic (stationary or mobile). Self-collected specimens cannot be used for cervical cytology because they do not sample the transformation zone of the cervix, which is a prerequisite for cytology.

The following consumables are required for self-collection of samples:

• Brush or swab for sample collection from the upper vagina
• Tube containing specimen transport medium
• Printed label or a pen to write on the specimen tube
• Laboratory requisition form

Brush or swab for self-collection of specimens for HPV testing: Special types of brushes or Dacron swabs are available for self-collection. Some of the devices have an indicator to help women understand how far the brush has to be inserted into the vagina. The Christmas-tree-shaped brush with soft bristles used for cervical specimen collection can also be used for self-collection.

The next section describes the steps for collection of cervical samples for HPV testing.